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JOURNAL ARTICLE
REVIEW
Engineering yeast for utilization of alternative feedstocks.
Current Opinion in Biotechnology 2018 January 11
Realizing the economic benefits of alternative substrates for commodity chemical bioproduction typically requires significant metabolic engineering of common model organisms, such as Saccharomyces cerevisiae. A growing toolkit is enabling engineering of non-conventional yeast that have robust native metabolism for xylose, acetate, aromatics, and waste lipids. Scheffersomyces stipitis was engineered to produce itaconic acid from xylose. Yarrowia lipolytica produced lipids from dilute acetate at over 100g/L. Cutaneotrichosporon oleaginosus was engineered to produce omega-3 fatty acids and recently was shown to accumulate nearly 70% lipids when grown on aromatics as a carbon source. Further improvement to toolkits for genetic engineering of non-conventional yeast will enable future development of alternative substrate conversion to biochemicals.
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