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Facilitating circular permutation using Restriction Free (RF) cloning.
Protein Engineering, Design & Selection : PEDS 2018 March 2
Circular permutation is a powerful tool to test the role of topology in protein folding and function. Previous methods for generating circular permutants were based on rearranging gene elements using restriction enzymes-based cloning. Here, we present a Restriction Free (RF) approach to achieve circular permutation which is faster and more cost-effective.
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