Evidence for passerine bird pollination in Rhododendron species.

When insect activity is limited at low temperature, birds may be comparatively more important pollinators than insects for flowering plants. It has been thought that many large-flowered Rhododendron species are pollinated by local birds in the Himalayan regions because most of these species flower in spring at high elevation with cool atmospheric temperature. However, experimental evidence for the role of bird pollination in this hyperdiverse genus remains scarce. To examine the role of birds and insects in pollination, we observed floral visitors to 15 Rhododendron species with different floral sizes and abundant flowering individuals in the eastern Himalayas, Southwest China. To examine the role of birds and insects in female reproductive success in each species, cages were used to exclude birds but not insects from visiting flowers and net bags were used to exclude all floral visitors. Inflorescences where visitation was excluded did not produce fruits in any of the Rhododendron species, indicating that sexual reproduction in these species depended on pollinator visitation. Bird visits were generally less frequent than bee visits in the studied species. However, in the nine species on which bird visitors were observed, fruit and/or seed set were greatly reduced in inflorescences caged to exclude birds but not bees, compared to open-pollinated inflorescences. In the other six species on which bird visitation was not observed, fruit and seed set did not differ significantly between caged and open inflorescences except in one species ( R. wardii ). Manipulations to achieve selective exclusion of visitors demonstrated that birds could be effective pollinators for 10 out of 15 studied Rhododendron species in the eastern Himalayas. Floral characteristics of these Rhododendron species and weather conditions might favour the evolution of bird pollination systems in the East Himalayas.