The Osteogenic Differentiation Effect of the FN Type 10-Peptide Amphiphile on PCL Fiber.

The fibronectin type 10-peptide amphiphile (FNIII10-PA) was previously genetically engineered and showed osteogenic differentiation activity on rat bone marrow stem cells (rBMSCs). In this study, we investigated whether FNIII10-PA demonstrated cellular activity on polycaprolactone (PCL) fibers. FNIII10-PA significantly increased protein production and cell adhesion activity on PCL fibers in a dose-dependent manner. In cell proliferation results, there was no effect on cell proliferation activity by FNIII10-PA; however, FNIII10-PA induced the osteogenic differentiation of MC3T3-E1 cells via upregulation of bone sialoprotein ( BSP ), collagen type I ( Col I ), osteocalcin ( OC ), osteopontin ( OPN ), and runt-related transcription factor 2 ( Runx2 ) mitochondrial RNA (mRNA) levels; it did not increase the alkaline phosphatase ( ALP ) mRNA level. These results indicate that FNIII10-PA has potential as a new biomaterial for bone tissue engineering applications.