Rose petal and P123 dual-templated macro-mesoporous TiO 2 for a hydrogen peroxide biosensor.

In this work, highly ordered macro-mesoporous TiO2 has been successfully synthesized using fresh rose petals and P123 (EO20 PO70 EO20 ) as dual templates through a simple soaking and calcining process. Characterization of the as-prepared TiO2 indicated that the mesoporous structure of the TiO2 was highly ordered, with a pore diameter of approximately 3nm. After electrodeposition of Pt nanoparticles onto the TiO2 as an electron transfer enhancer and the immobilization of horseradish peroxidase (HRP) onto the TiO2 -modified electrode, a biosensor for detecting hydrogen peroxide (H2 O2 ) was realized. This biosensor showed a wide linear detection range from 5μM to 8mM and a low detection limit of 1.65μM with good stability and high selectivity, suggesting that the sensor is well-suited for the detection of H2 O2 .