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Response to Letter to the Editor entitled 'Correct microarray analysis approaches in "Hsa-circRNA11783-2 in peripheral blood is correlated with coronary artery disease and type 2 diabetes mellitus"'.
Diabetes & Vascular Disease Research 2018 March
In the paper entailed 'Hsa-circRNA11783-2 in peripheral blood is correlated with coronary artery disease and type 2 diabetes mellitus', we illustrated the correlation of circRNA with T2DM and CAD. We used the common method - Q-PCR to select differentially expressed circular RNA (differentially expressedgenes(DEGs)), with a criteria of fold change>2 and unadjusted p values < 0.05 in expression. When the candidate RNAs have been narrowed down by GO and Pathway analysis, Q-PCR is a good verification method, although the idea that using Q-PCR to verify all the DEGs is still unrealistic. There is no denying that using an optimal statistical approach makes the survey much easier.
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