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New Transcriptional Reporters to Quantify and Monitor PPAR γ Activity.
PPAR Research 2017
The peroxisome-proliferator-activated-receptor- γ (PPAR γ ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPAR γ , we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPAR γ activity in different cell types and screen for PPAR γ 's potential ligands.
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