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[DNA Extraction from Old Bones by AutoMate Express™ System].

Fa Yi Xue za Zhi 2017 August
OBJECTIVES: To establish a method for extracting DNA from old bones by AutoMate Express™ system.

METHODS: Bones were grinded into powder by freeze-mill. After extraction by AutoMate Express™, DNA were amplified and genotyped by Identifiler®Plus and MinFiler™ kits.

RESULTS: DNA were extracted from 10 old bone samples, which kept in different environments with the postmortem interval from 10 to 20 years, in 3 hours by AutoMate Express™ system. Complete STR typing results were obtained from 8 samples.

CONCLUSIONS: AutoMate Express™ system can quickly and efficiently extract DNA from old bones, which can be applied in forensic practice.

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