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Total Internal Reflection Fluorescence Microscopy to Study GLUT4 Trafficking.

Sebastian Wasserstrom, Björn Morén, Karin G Stenkula
Methods in Molecular Biology 2018
Total internal reflection fluorescence (TIRF) microscopy is a powerful method that allows examination of plasma membrane close events in real time. The last decade, the method has successfully been used to explore GLUT4 translocation in adipocytes. Here, we describe the procedure for studying GLUT4 trafficking using TIRF microscopy in isolated primary adipocytes.

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