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A broad-host range dual-fluorescence reporter system for gene expression analysis in Gram-negative bacteria.
Journal of Microbiological Methods 2018 January
Fluorescence-based reporter systems are valuable tools for studying gene expression dynamics in living cells. Here we describe a dual-fluorescence reporter system carrying the red fluorescent marker mCherry and the blue fluorescent protein EBFP2 enabling the simultaneous analysis of two promoters in broad-host range autofluorescent Gram-negative bacteria.
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