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Isolation and characterization of mesenchymal stem cells and its antitumor application on ovarian cancer cell line.
Artificial Cells, Nanomedicine, and Biotechnology 2017 December 4
The molecular interaction network of Oct-4 (POU5F1) and NANOG connected to regulation and growth of mesenchymal stem cells (MSCs) were supplemented with information of miRNA to find an important micro-RNAs and supplemented molecular interaction network. Following co-culturing of Bone marrow mesenchymal stem cells (BMMSCs) with SKOV3 ovarian cancer cell lines and undifferentiated BMMSCs, MTT was analyzed for cell cytotoxicity. The analyses of the expression of miRNA were performed either after oesteogenesis (hsa-miR-34 and hsa-miR-335) or chondrogenic (hsa-miR-145 and hsa-miR-455) differentiation. This molecular interaction network was imaged in using software. The results from these findings gave an understanding of the main molecular mechanisms regulating MSCs therapeutic activity and their undifferentiated state maintenance. We recommend that the downregulation of miR-335 is crucial role for tissue homeostasis.
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