A New Dihydrochromone Dimer and Other Secondary Metabolites from Cultures of the Marine Sponge-Associated Fungi Neosartorya fennelliae KUFA 0811 and Neosartorya tsunodae KUFC 9213.

A previously unreported dihydrochromone dimer, paecilin E ( 1 ), was isolated, together with eleven known compounds: β-sitostenone, ergosta-4,6,8 (14), 22-tetraen-3-one, cyathisterone, byssochlamic acid, dehydromevalonic acid lactone, chevalone B, aszonalenin, dankasterone A ( 2 ), helvolic acid, secalonic acid A and fellutanine A, from the culture filtrate extract of the marine sponge-associated fungus Neosartorya fennelliae KUFA 0811. Nine previously reported metabolites, including a chromanol derivative ( 3 ), (3β, 5α, 22 E ), 3,5-dihydroxyergosta-7,22-dien-6-one ( 4 ), byssochlamic acid, hopan-3β,22-diol, chevalone C, sartorypyrone B, helvolic acid, lumichrome and the alkaloid harmane were isolated from the culture of the marine-sponge associated fungus Neosartorya tsunodae KUFC 9213. Paecilin E ( 1 ), dankasterone A ( 2 ), a chromanol derivative ( 3 ), (3β, 5α, 22 E )-3,5-dihydroxyergosta-7,22-dien-6-one ( 4 ), hopan-3β,22-diol ( 5 ), lumichrome ( 6 ), and harmane ( 7 ) were tested for their antibacterial activity against Gram-positive and Gram-negative reference and multidrug-resistant strains isolated from the environment. While paecilin E ( 1 ) was active against S. aureus ATCC 29213 and E. faecalis ATCC 29212, dankastetrone A ( 2 ) was only effective against E. faecalis ATCC 29212 and the multidrug-resistant VRE E. faecalis A5/102. Both compounds neither inhibit biofilm mass production in any of the strains at the concentrations tested nor exhibit synergistic association with antibiotics.