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Multichannel Dynamic Interfacial Printing: An Alternative Multicomponent Droplet Generation Technique for Lab in a Drop.
ACS Applied Materials & Interfaces 2017 December 21
Generation of uniform emulsion droplets mixed with multiple components is one of the key issues in the field of lab in a drop. Traditionally, droplet microfluidic chips are often served as the prime choice while designing and fabricating microfluidic chips always rely on skilled technician and specialized equipment, severely restricting its wide accessibility. In this work, an alternative technique, called multichannel dynamic interfacial printing (MC-DIP), was proposed for multicomponent droplet generation. The MC-DIP device was designed modularly and could be set up manually without any microfabrication process, exhibiting full accessibility for freshmen after a brief training. This new technique owns advantages in the generation of droplets with predictable sizes and composites. Quantitative experiments of measuring minimum inhibitory concentration (MIC) value via mixing microbes and antibiotics into droplet were conducted to proving its application potential for lab in a drop. Further research on a clinical pathogenic strain revealed that this technique could be potentially applied in the clinical laboratory for antibiotic susceptibility testing.
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