Enhanced phenanthrene degradation in river sediments using a combination of biochar and nitrate.

Polycyclic aromatic hydrocarbons (PAHs) pollution in urban river sediments is a serious problem to ecological systems and human health. We examined novel remediation approaches, using a biochar amendment combined with bioaugmentation or/and nitrate stimulation, to degrade phenanthrene in sediment. Biochar amendment combined with nitrate stimulation enhanced phenanthrene degradation by 2.3 times that of the control and 1.9 times that of biochar alone. Nitrate stimulation altered the microbial succession and encouraged the growth of potential nitrate-reducing PAH-degraders Thiobacillus and Stenotrophomonas. Biochar was an excellent sorbent for phenanthrene and the shelter that it provided PAH-degraders increased contact between phenanthrene and PAH-degraders. Biochar also enhanced the aging effects of phenanthrene and reduced the ecological risk by 7.7% to 11%. These results suggest that biochar amendment combined with nitrate stimulation can achieve high-efficiency phenanthrene degradation in sediments.