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Direct measurement of the mechanism by which magnesium specifically modifies the mechanical properties of DNA.
Biomicrofluidics 2017 September
We examine the effect of physiological cations Na+ , K+ , Mg2+ , and Ca2+ on the mechanical properties of bundles of λ-phage DNA using silicon nanotweezers (SNTs). Integrating SNTs with a microfluidic device allows us to perform titration experiments while measuring the effect in real-time. The results show that only for Mg2+ and in particular, at the intra-nuclear concentration (100 mM), the interaction occurs.
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