Effects of 60-Week Feeding Diet Containing Bt Rice Expressing the Cry1Ab Protein on the Offspring of Inbred Wuzhishan Pigs Fed the Same Diet.

We evaluated the chronic effects of Bt rice carrying the Cry1Ab protein (1.64 mg/kg) on offspring of highly inbred WZSP, fed with Bt rice for 360 days, in a 60-week feeding study. The WZSP offspring (n = 27) were assigned to two groups (Minghui86 group, female n = 6, male n = 5; Bt group, female n = 11, male n = 5). The average obtained Cry1Ab protein dosage for female and male pigs was 1.003 and 1.234 mg/kg body weight after 10 weeks of feeding, respectively. The experimental feed in the study was nutritionally matched in both groups. The average daily gain and feed conversion ratio of the females in week 3 and males from weeks 1 to 10 were different between the Bt and Minghui86 groups (P < 0.05), and the body weight of the male pigs in week 2 was greater in the Minghui86 group than that of the Bt group (P < 0.05). No other differences were observed, and there were no significant differences in the serum sex steroid level, hematology parameters, relative organ weights, or histopathology. Although differences in some serum chemistry parameters (alanine aminotransferase of female pigs and alkaline phosphatase of male pigs) were observed, they were not considered treatment-related. On the basis of these results, long-term intake of transgenic rice carrying Cry1Ab protein exerts no unintended adverse effects on WZSP offspring.