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Applying inbreeding, hybridization and mutagenesis to improve oxidative stress tolerance and longevity of the entomopathogenic nematode Heterorhabditis bacteriophora.
Journal of Invertebrate Pathology 2018 January
Poor shelf-life and sensitivity to environmental stress of entomopathogenic nematodes (EPNs) are traits, which deserve attention for improvement. Recently, a strong positive correlation between oxidative stress tolerance and longevity of Heterorhabditis bacteriophora dauer juveniles (DJs) has been reported. In this study, the improvement of H. bacteriophora DJ longevity was achieved by hybridization and mutagenesis. A hybrid pool deriving from two oxidative stress tolerant and long-living parental strains was generated. This hybrid AU1 × HU2 survived 2.6 days and 18 days longer than its best parent under oxidative stress and control conditions, respectively. In addition to the natural genetic variability, an EMS-mutant pool (M-OXI) with high longevity was generated and one of the derived mutagenized inbred lines (MOX-IL6) survived 5.8 days and 28.4 days longer than its donor line (IL3) under oxidative stress and control conditions, respectively. A genetic cross between the mutagenized inbred line and its donor line (MOX-IL × IL3) still survived 2.5 days and 18.5 days longer than the donor line under oxidative stress and control conditions, respectively. Concerning virulence and reproductive potential, trade-off effects were not observed as a result of hybridization and mutagenesis. These results underline the potential of classical genetic approaches for trait improvement in the nematode H. bacteriophora.
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