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Evaluation of ATR-FTIR for analysis of bacterial cellulose impurities.
Journal of Microbiological Methods 2018 January
This research evaluated the utility of using the large amount of spectral data obtained during attenuated total reflection Fourier-transform infrared spectrophotometry (ATR-FTIR) analysis of dried biocellulose (BC) to estimate the type and concentration of potential bacterial cells impurities present in the BC. Pre-cleaned BC was contaminated with known concentrations of representative nucleic acid, lipid, and protein impurities, as well as whole bacterial cells. These impurity standards were then subjected to ATR-FTIR analysis, and the resulting spectral data were used to develop models to estimate the concentrations of impurities in differentially processed BC. Results indicated that ATR-FTIR is a useful tool for estimating impurities in BC, and may also be applicable for measurement of levels of non-cellulose biomolecules added to BC for various purposes.
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