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A case report of personal exposures to isoflurane during animal anesthesia procedures.
Journal of Occupational and Environmental Hygiene 2018 Februrary
The purpose of this study was to compare personal exposures to isoflurane from participants' breathing zone samples during animal anesthesia procedures by the method of anesthetic gas delivery and the waste anesthetic gas (WAG) control method utilized. WAG control methods included passive scavenging using charcoal canisters, active scavenging using a building vacuum system, and various local exhaust ventilation systems such as laboratory fume hoods and capture hoods. Methods of anesthesia delivery included induction chambers, face masks (also known as nose cones), and intubation. Personal breathing zone samples were collected using 3M 3520 Organic Vapor Diffusion Monitors and submitted to an International Organization for Standardization (ISO) 17025 accredited laboratory for analysis. When using face masks and induction chambers as the method of anesthesia delivery, local exhaust ventilation systems were found to be the best WAG control method to mitigate personal exposures to isoflurane. Personal exposures to isoflurane were well-controlled when animals were intubated, regardless of whether passive scavenging with an adsorptive charcoal canister or active scavenging with a building vacuum system was used. Personal exposures to isoflurane were highest when induction chambers and face masks were used for anesthesia delivery, and passive scavenging with adsorptive charcoal canisters were used as the control method. This study served to identify best practice WAG control methods for research and veterinary procedures that involve isoflurane anesthesia.
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