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[Main Factors Influencing FeCl 3 -Induced Mouse Mesenteric Arteriole Thrombosis Model].
Zhongguo Shi Yan Xue Ye Xue za Zhi 2017 October
OBJECTIVE: To investigate the factors that influence FeCl3 -induced mouse mesenteric arteriole thrombosis model.
METHODS: Platelets were isolated from donor mice and labeled with Calcein-AM. Mice were transfused intravenously with Calcein-AM labeled platelets. The influence of mouse ages (3-6 weeks, 6-10 weeks and >10 weeks), transfused platelets counts (1×107 , 1×108 and 2×108 platelets) and concentrations of FeCl3 (6%, 12%, 24% and 48%) on FeCl3 -induced thrombosis model were compared.
RESULTS: The occlusion time was 16 min for mice aged 3-6 weeks, which was shorter than that for 6 mice aged 6-10 weeks(25 min)(P<0.05) and that for mice aged >10 weeks(38 min)(P<0.01). The occlusion time resulting from transfusion of 1×108 and 2×108 of pletclets was 15-18 min, which was shorter than that of transfusion 1×107 platelets (30 mins). The occlusion time resulting from transfusion of 6% and 12% FeCl3 was from 15 to 20 min, however the transfusion of 24% and 48% FeCl3 all in all leads to vessel occlusion within 10 min.
CONCLUSION: The factors influencing the success of FeCl3 -induced mouse thrombosis model are more. Transfusion of 1×108 to 2×108 labeled platelets to 3-6 week-old mice, and 6% to 12% of FeCl3 should be used to induce thrombosis, and the experimental conditions should be optimized for this animal model, therefore, it is easier for us to set up a mouse mesenteric arteriole thrombosis model.
METHODS: Platelets were isolated from donor mice and labeled with Calcein-AM. Mice were transfused intravenously with Calcein-AM labeled platelets. The influence of mouse ages (3-6 weeks, 6-10 weeks and >10 weeks), transfused platelets counts (1×107 , 1×108 and 2×108 platelets) and concentrations of FeCl3 (6%, 12%, 24% and 48%) on FeCl3 -induced thrombosis model were compared.
RESULTS: The occlusion time was 16 min for mice aged 3-6 weeks, which was shorter than that for 6 mice aged 6-10 weeks(25 min)(P<0.05) and that for mice aged >10 weeks(38 min)(P<0.01). The occlusion time resulting from transfusion of 1×108 and 2×108 of pletclets was 15-18 min, which was shorter than that of transfusion 1×107 platelets (30 mins). The occlusion time resulting from transfusion of 6% and 12% FeCl3 was from 15 to 20 min, however the transfusion of 24% and 48% FeCl3 all in all leads to vessel occlusion within 10 min.
CONCLUSION: The factors influencing the success of FeCl3 -induced mouse thrombosis model are more. Transfusion of 1×108 to 2×108 labeled platelets to 3-6 week-old mice, and 6% to 12% of FeCl3 should be used to induce thrombosis, and the experimental conditions should be optimized for this animal model, therefore, it is easier for us to set up a mouse mesenteric arteriole thrombosis model.
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