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Capillary electrophoresis separation of phenethylamine enantiomers using amino acid based ionic liquids.
Journal of Pharmaceutical and Biomedical Analysis 2018 January 31
In recent years increasing interest was drawn towards ionic liquids in analytical separation science, such as capillary electrophoresis. Ionic liquids combining tetrabutylammonium cations with chiral amino acid based anions were prepared and investigated as capillary electrophoresis background electrolyte additives for the enantioseparation of ephedrine, pseudoephedrine, and methylephedrine isomers. For the optimization of buffer pH and ionic liquid concentration a design of experiments approach was performed. The best results for the separation of all enantiomers were achieved using 125mmol/L tetrabutylammonium l-argininate in a 75mmol/L phosphate buffer pH 1.5 containing 30mmol/L β-cyclodextrin.
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