Exfoliative Endometrial Cytology in Embryo Donor Cows-Comparison of Sampling Localizations for the Diagnosis of Subclinical Endometritis.

Subclinical endometritis has a major effect on the reproductive performance of dairy cows and also on the success of embryo collection. Thus it is important to minimize the number of false-negative diagnoses. In order to evaluate the question of whether or not a single cytobrush sample is representative of the whole endometrium, 53 German Holstein embryo donor cows in the northwest of Germany were examined via the cytobrush method at three different localizations of the uterus: the uterine body about 0.5 cm cranial of the cervical canal and both uterine horns about 1.5 cm cranial of the bifurcation. Although the mean percentage of polymorphonuclear neutrophils at the three locations is not significantly different ( p = 0.64), the individual variations lead to the conclusion that more than one sample of the endometrium should be taken into account when diagnosing subclinical endometritis in embryo donor cows.