Paraferrimonas haliotis sp. nov., isolated from the intestine of abalone, Haliotis discus hannai and emendation of description of the genus Paraferrimonas.

Two strains, AFRC7-2-1T and CFD7-4-2, were isolated from the intestine of abalone, Haliotis discus hannai. The bacterial cells were Gram-stain-negative, stick-shaped and non-flagellated. They were facultative anaerobic bacteria and could reduce Fe(III) to Fe(II). The 16S rRNA genes of two strains shared 99.7 % sequence similarity, and had the highest similarity of 97.3-97.5 % with Paraferrimonas sedimenticola NBRC 101628T , and of <94.0 % with other species. Phylogenetic relationship showed that the two strains formed a tight clade with an isolate Paraferrimonas sp. CGB11 obtained from the intestine of small abalone, H. diversicolor, and P. sedimenticola NBRC 101628T . The resulted DNA-DNA hybridization (DDH) values of strains AFRC7-2-1T and CFD7-4-2 compared with P. sedimenticola NBRC 101628T was 18.8-18.9 %. The resultant average nucleotide identity (ANI) values of AFRC7-2-1T and CFD7-4-2 compared with P. sedimenticola NBRC 101628T was 71.3-71.5 %. The predominant fatty acids (>5 %) of strains AFRC7-2-1T and CFD7-4-2 were iso-C15 : 0, iso-C13 : 0, C14 : 0, C16 : 0, C17 : 1ω8c and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The quinone system of strains AFRC7-2-1T and CFD7-4-2 were quinone-8, menaquinone-6, menaquinone-7, and quinone-7. The predominant polar lipids of strain AFRC7-2-1T were identified as phosphatidylethanolamine (PE), phosphatidylglycerol (PG), one unidentified phospholipid (PL), and three minor unidentified lipids (Ls). The genomic DNA G+C contents of two strains were 47.2 mol%. In summary, the two strains represented a novel species of the genus Paraferrimonas, for which the name Paraferrimonas haliotis sp. nov. was proposed, with type strain AFRC7-2-1T (=MCCC 1A11748T =KCTC 52632T =NBRC 112785T ) and strain CFD7-4-2 (=MCCC 1A11749=KCTC 52631=NBRC 112786).