Influence of dental materials on cells of the equine periodontium.

BACKGROUND: Therapy for equine periodontal disease can include filling of the periodontal pockets and widened interproximal spaces. Recommended dental materials are generally adopted from human dentistry.

OBJECTIVES: To evaluate the biocompatibility of dental materials for equine periodontal fillings in vitro.

STUDY DESIGN: In vitro experiments.

METHODS: Four different dental materials (PeriCare® , Provicol® , Calxyl® and Honigum) were tested on equine periodontal fibroblasts. Possible cytotoxic effects were assessed microscopically and by MTT assay, and the expression of inflammatory marker genes was measured by qRT-PCR.

RESULTS: PeriCare® and Provicol® had no effects on the cells, whereas Honigum and Calxyl® were associated with severe cytotoxic effects.

MAIN LIMITATIONS: The results of this in vitro study need to be confirmed by clinical studies.

CONCLUSIONS: Before adapting dental materials from human dentistry, it is crucial to initially test them in a specific equine model.