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Journal Article
Research Support, Non-U.S. Gov't
Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers.
ChIP-seq is the current method of choice for genome-wide protein location analysis. Here, we present a native (non-cross-linked) ChIP procedure suitable for histone proteins, coupled with an efficient library preparation technique for subsequent next-generation sequencing. The method enables ChIP-seq starting with 50,000 or more cells.
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