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Labelling HaloTag Fusion Proteins with HaloTag Ligand in Living Cells.

Bio-protocol 2017 September 6
HaloTag has been widely used to label proteins in vitro and in vivo (Los et al., 2008). In this protocol, we describe labelling HaloTag-Cbx fusion proteins by HaloTag ligands for live-cell single-molecule imaging (Zhen et al., 2016).

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