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Research Support, Non-U.S. Gov't
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Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9.

Yingying Xu, Yuhang Wu, Dongsheng Guo, Ge Gao, Keyu Lai, Fan Yang, Kepin Wang, Han Wu, Liangxue Lai, Jialiang Li, Kecheng Xu, Yin-Xiong Li
Stem Cell Research 2017 July
The gene asialoglycoprotein receptor 1 (ASGR1) encodes a subunit of the asialoglycoprotein receptor. Here we report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37bp deletion, resulting in a frame shift. The homozygous knockout WA01 cell line maintains a normal karyotype, typical stem cell morphology, pluripotency and differentiation potential in vitro.

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