Effects of Glutathione S-Transferase Gene Polymorphisms and Antioxidant Capacity per Unit Albumin on the Pathogenesis of Chronic Obstructive Pulmonary Disease.

OBJECTIVES: To study the effects of GSTM 1, GSTT 1 gene polymorphisms, and organism antioxidant capacity and related indicators such as antioxidant capacity per unit of albumin (AC/ALB) on chronic obstructive pulmonary disease (COPD).

METHODS: Using polymerase chain reaction technology, GSTM 1 and GSTT 1 gene polymorphisms were detected in 33 COPD patients and 33 healthy people. The total antioxidant capacity (TAC) found in serum was determined using the I2 /KI potentiometric, KMnO4 microtitration, and H2 O2 potentiometric methods. The AC/ALB was defined as the TAC divided by the serum albumin concentration. Logistic regression analysis was carried out with biochemical screening indices, which was found to be closely related with the incidence of COPD.

RESULTS: The GSTM 1 and GSTT 1 gene deletion rate in the COPD group was significantly higher than that in the control group ( P < 0.05). The differences in serum TAC between the COPD and control groups, GSTM 1 (+) and GSTM 1 (-) groups, and GSTT 1 (+) and GSTT 1 (-) groups were statistically significant ( P < 0.001). In addition, there was a significant difference in the AC/ALB between the COPD and control groups ( P < 0.05). Logistic regression analysis showed that the incidence of COPD was closely related to the AC/ALB ( P < 0.05).

CONCLUSIONS: GSTM 1 and GSTT 1 gene polymorphisms are closely correlated with the pathogenesis of COPD, while the AC/ALB plays a decisive role in the occurrence and development of COPD.