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JOURNAL ARTICLE
VALIDATION STUDIES
Development and validation of a high-throughput method for determination of nine fluoroquinolones residues in muscle of different animal species by liquid chromatography coupled to tandem mass spectrometry with low temperature clean up.
Journal of Chromatography. A 2017 October 28
This work describes the development and validation of a quantitative and confirmatory method to determination of nine fluoroquinolones residues in poultry, bovine, swine and fish muscle using LC-MS/MS. Sample preparation was based in a fast solvent extraction with acetonitrile with 1% of formic acid followed by a low-temperature clean up procedure and centrifugation, without further steps. The recoveries ranged between 79% and 115%. The concentration work range was 0-200μgkg-1 . The LOD and LOQ were 5 and 10μgkg-1 , respectively. This high-throughput method was reliable for identification and confirmation of nine relevant compounds in food-producing animal tissues. Validation procedure was performed according the Directive 2002/657/EC criteria and the method showed fitness to purpose in terms of precision and reproducibility as well as for the other performance parameters.
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