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The derivatization and antioxidant activities of yeast mannan.
International Journal of Biological Macromolecules 2018 Februrary
The alkaline mannan (M) was extracted from yeast cell walls, and the best extraction condition was: 100°C, 2h, 1% NaOH, solid-liquid ratio of 10:1. The purity of mannan was 96.15%. Its five derivatives were obtained by chemical modification methods, and they were: phosphorylated mannan (P-M), sulfated mannan (S-M), carboxymethylated mannan (CM-M), carboxymethylated-phosphorylated mannan (CMP-M), and carboxymethylated-sulfated mannan (CMS-M). The phosphate substitution degree of P-M and CMP-M was 0.2 and 0.1, respectively. The sulfate substitution degree of S-M and CMS-M was 0.67 and 0.65, respectively. The carboxymethyl substitution degree of CM-M, CMP-M and CMS-M were 1.11, 1.17 and 0.25, respectively. It indicated that the hydroxyl radical scavenging capacities of P-M and CMP-M were 15% higher than that of M. The anti-lipid peroxidation capacity of all derivatives increased than that of M, especially for CMP-M, its scavenging effect was 25% higher.
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