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Journal Article
Research Support, N.I.H., Extramural
Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography.
Applied Radiation and Isotopes 2017 December
Positron Emission Tomography is an attractive imaging modality for monitoring the migration of cells to pathological tissue. We evaluated a new method for radiolabelling leukocytes with zirconium-89 (89 Zr) using chitosan nanoparticles (CN, Z-average size 343 ± 210nm and zeta potential +46 ± 4mV) as the carrier. We propose that cell uptake of 89 Zr-loaded CN occurred in a two-step process; cell membrane interaction with 89 Zr-loaded CN was followed by a slower cell internalisation step.
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