Nocardioides kandeliae sp. nov., an endophytic actinomycete isolated from leaves of Kandelia candel.

A Gram-stain-positive, aerobic, non-spore-forming, atrichous and short rod-shaped endophytic actinomycete, designated strain BGMRC 2075(T), was isolated from the leaves of Kandelia candel, and was subjected to polyphasic characterization to unravel its taxonomic position. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BGMRC 2075(T) belongs to the genus Nocardioides ,showing the highest 16S rRNA gene sequence similarity to Nocardioides aestuarii JC2056(T) (96.1 %), Nocardioides agariphilus MSL-28(T) (95.1 %) andNocardioides islandiensis MSL-26(T) (95.1 %). The predominant cellular fatty acids of strain BGMRC 2075(T) were iso-C16 : 0, C17 : 1ω8c and C17 : 0. The major menaquinone was MK-8(H4). The diagnostic diamino acid in the cell-wall peptidoglycan was ll-2,6-diaminopimelic acid. The predominant cell-wall sugars were composed of ribose and glucose. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, five unknown phospholipids, one phospholipid of unknown structure containing glucosamine, and an unknown polar lipid. The DNA G+C content was 70.8 mol%. All these data support the allocation of the novel strain to the genus Nocardioides. The results of physiological and biochemical characterization allow the phenotypic differentiation of strain BGMRC 2075(T) from N. aestuarii JC2056(T), N. agariphilus MSL-28(T) and N. islandiensis MSL-26(T). Strain BGMRC 2075(T) represents a novel species of the genus Nocardioides, for which we propose the name Nocardioides kandeliae sp. nov. The type strain is BGMRC 2075(T) (=KCTC 39886(T)=DSM 104480(T)).