Croceivirga radicis gen. nov., sp. nov., isolated from a rotten tropical mangrove root.

A novel bacterial strain, designated HSG9(T), was isolated from aisolated from a rotten tropical mangrove root. Cells of strain HSG9(T) were aerobic, Gram-stain-negative, yellow, oxidase-negative and catalase-positive. Growth was observed in 0.5-9 % sea salt (optimum 3 %, w/v), at 10-42 °C (optimum 25-35 °C) and at pH 6.0-8.0 (optimum 7.0-8.0). Gelatin, esterase and Tweens 20, 40, 60 and 80 were hydrolysed, but starch, protein, cellulose and casein were not. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain HSG9(T) formed an independent lineage related to the family Flavobacteriaceae. The dominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G. The respiratory quinone was identified as MK-6 and the polar lipids were phosphatidylethanolamine, three unidentified phospholipids and an unidentified lipid. The DNA G+C content was 37.1 mol%. The combined genotypic and phenotypic data indicated that strain HSG9(T) represents a novel species of a new genus within the family Flavobacteriaceae, for which the name Croceivirgaradicis gen. nov., sp. nov. is proposed. The type strain is HSG9(T) (=MCCC 1A06690(T)=KCTC 52589(T)).