Spike propagation in axons under stretch growth conditions in cultured neurons from dorsal root ganglion.

Computational software NEURON was used to simulate the stretch growth neurons in order to investigate the ability of dorsal root ganglion neurons to generate and propagate action potentials after a period of rapid axon stretch growth in vitro, and under what stimulating parameters can evoke action potentials. In the simulation, we found the stretch growth neuron had higher spike amplitude than from the static culture neuron in the soma and all axonal branch. In addition, the conduction velocity was also faster in the stretch growth axon. When the stimulating frequency was less than 15 Hz or the stimulating voltage was lower than 15 mV, no spike was evoked. Increasing stimulating frequency from 15 Hz to 5000 Hz or stimulating voltage from 15 mV to 100 mV had almost no effect on the spike amplitude. Interestingly, the first spike time and absolute refractory period (ARP) in different axonal branches and somas decreased stepwise with incremental increase in the stimulating frequency. It is concluded that the stretch growth neuron had higher amplitude and faster conduction velocity than the static culture neuron. In addition, some stimulating parameters had been analyzed in this study, which provided guidelines for electrophysiological experiments in future.