Calorespirometry: A Novel Tool in Functional Hologenomics to Select "Green" Holobionts for Biomass Production.

Endophytes can diversify temperature response and biomass production in plants and microalgae. Natural and inoculated endophytes that modify growth performance are increasingly considered in research and practical initiatives for sustainable agriculture. However, efficient, novel tools are required that are able to support identification of differential effects of native endophyte populations and for pre-selection of inocula.This protocol gives instructions for applying calorespirometry as a rapid means for identifying differential effects of endophytes on temperature response and predicted biomass productivity in microalgae and plant holobionts. The protocol can help discriminating hologenomes, genes, and molecular neutral or functional markers for microalgae strain and plant improvement. Here, we focus on the microalga Chlorella vulgaris and associated microorganisms as an example for highlighting the methodology for its integration in research and application.