Interferon-γ suppresses the proliferation and migration of human placenta-derived mesenchmal stromal cells and enhances their ability to induce the generation of CD4 + CXCR5 + Foxp3 + Treg subset.

We investigate the effects of interferon (IFN)-γ on human placenta-derived mesenchymal stromal cells (hPMSCs), in particular, their adhesion, proliferation and migration and modulatory effects on the CD4+ CXCR5+ Foxp3+ Treg subset. And we compared hPMSCs ability to induce the generation of different Treg subsets in response to treatment with IFN-γ. We found that IFN-γ suppressed the proliferation and migration for hPMSCs. The ability of hPMSCs to induce the generation of CD4+ CXCR5+ Foxp3+ Treg subset was enhanced by IFN-γ. And maximal effectiveness of IFN-γ treated hPMSCs upon inducing the generation of Treg subsets was for CD4+ CXCR5+ Foxp3+ Treg subset as compared with that of CD4+ CD25+ Foxp3+ , CD8+ CD25+ Foxp3+ , CD4+ IL-10+ and CD8+ IL-10+ Treg subsets. These results have important implications for the development and application of hPMSCs in clinical use.