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Rodent-free cyclical transmission of Trypanosoma brucei brucei.

Arunasalam Naguleswaran, Isabel Roditi
Molecular and Biochemical Parasitology 2017 October
We provide a simple protocol enabling cyclical transmission of Trypanosoma brucei brucei to be performed without the need for mammals. These procedures have two advantages: they are in line with 3R principles of animal use - replace, refine, reduce - and may enable more laboratories to study the complete life cycle.

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