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Draft genome sequence of Enterobacter cloacae ST520 harbouring blaKPC-2, blaCTX-M-15 and blaOXA-17 isolated from coastal waters of the South Atlantic Ocean.
Journal of Global Antimicrobial Resistance 2017 September
OBJECTIVES: Aquatic environments have contributed to the dissemination of multidrug-resistant (MDR) bacteria, representing a risk for humans and animals. The aim of this study was to report the first draft genome sequence of a MDR Enterobacter cloacae strain recovered from seawater in a public beach in Brazil.
METHODS: The genome was sequenced on an Illumina MiSeq platform. De novo genome assembly was performed using SPAdes 3.10.1 and the whole genome sequence was analysed using bioinformatics tools from the Center of Genomic Epidemiology.
RESULTS: This draft genome resulted in 5 228 857bp with 5331 protein-coding sequences, revealing the presence of blaKPC-2, blaCTX-M-15 and blaOXA-17 genes, responsible for resistance to all β-lactam antibiotics. In addition, the strain was assigned to sequenced type 520 (ST520).
CONCLUSION: These data provide useful information for comparative genomic analysis regarding the dissemination of antibiotic resistance genes.
METHODS: The genome was sequenced on an Illumina MiSeq platform. De novo genome assembly was performed using SPAdes 3.10.1 and the whole genome sequence was analysed using bioinformatics tools from the Center of Genomic Epidemiology.
RESULTS: This draft genome resulted in 5 228 857bp with 5331 protein-coding sequences, revealing the presence of blaKPC-2, blaCTX-M-15 and blaOXA-17 genes, responsible for resistance to all β-lactam antibiotics. In addition, the strain was assigned to sequenced type 520 (ST520).
CONCLUSION: These data provide useful information for comparative genomic analysis regarding the dissemination of antibiotic resistance genes.
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