We have located links that may give you full text access.
Activity-guided isolation and structural identification of immunomodulating substances from Pleurotus eryngii byproducts.
International Immunopharmacology 2017 October
A two-step extraction process was used to investigate the bioactive substances from the byproducts of the fungus Pleurotus eryngii. Methanol extraction followed by extraction using different polar solvents coupled with silica column chromatography was carried out to separate the bioactive components. The immunomodulating activity of the extracts was investigated using Ana-1 mouse macrophage cells. At 25μg/mL, the butyl alcohol extract showed the highest enhancement of Ana-1 cell proliferative activity and secretion of cytokines. Using silica-gel column chromatography, the butyl alcohol extract was purified to seven elution fractions. Fr. 2 showed the highest promotion of Ana-1 macrophage cell proliferation and TNF-α, IL-6 secretion. HPLC-QTOF-MS/MS was used to determine the structure of the active substance in Fr. 2. Adenosine was identified as the primary active component in Fr. 2 with a chemical formula of C10 H13 N5 O4 . Adenosine may be useful as a natural immunostimulatory agent and anti-tumor agent.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app