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Highly selective on-off fluorescence recognition of Fe 3+ based on a coumarin derivative and its application in live-cell imaging.

A novel coumarin chemosensor, 7-hydroxy-2-oxo-N-(pyridin-2-ylmethyl)chromene-3-carboxamide (Probe 1), demonstrated significant selectivity towards Fe3+ ions. Probe 1 exhibited high fluorescence emission profile at 447nm, excellent selectivity towards Fe3+ over other biologically important metal ions (Al3+ , Ba2+ , Co2+ , Cu2+ , Zn2+ , Cd2+ , Hg2+ , Pb2+ and Sn2+ ). Interestingly, there was ~30-fold decrease in fluorescence intensity upon Fe3+ binding. The limit of detection of Fe3+ was found to be 0.76μM (~40ppb). Probe 1 also exhibited high potential as an intracellular chemosensor for Fe3+ .

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