Rational Design of GO-Modified Fe 3 O 4 /SiO 2 Nanoparticles with Combined Rhenium-188 and Gambogic Acid for Magnetic Target Therapy.

Peanutlike magnetic-fluorescent Fe3 O4 /SiO2 nanoparticles, with an effective dynamic diameter of 180 nm, were synthesized via EuO+ doping and coupling of two Fe3 O4 cores and reassembling through the solvothermal process. Spherical pure Fe3 O4 /SiO2 nanoparticles with an effective dynamic diameter of 230 nm were also prepared for comparison. We designed graphene oxide (GO)-modified core-shell Fe3 O4 /SiO2 nanoparticles as a nanocarrier for loading gambogic acid (GA) following labeling with radioisotope rhenium-188. We also performed GA loading and releasing on GA-loaded magnetic nanoparticles, in vivo biodistribution, and magnetic drug targeting therapy experiments. Results indicated that the GA-loaded magnetic nanoparticles demonstrate a clear pH-dependent drug release behavior, having a higher release rate in acidic environments. The in vivo biodistribution of the magnetic nanoparticles has morphologic dependency, and the peanutlike nanoparticles (PN-Fe3 O4 ) tend to accumulate more in the spleen, lung, and liver than in the spherical nanoparticles (S-Fe3 O4 ). The targeted therapy showed a higher efficacy of PN-Fe3 O4 in inhibiting tumor cell growth than the nontargeted therapy. The polyethyleneimine (PEI) grafting of PN-Fe3 O4 with amide bond was also designed to find an effective active targeting antitumor agent considering the fact that the PEI-GO conjugate has a higher GA load efficiency and the convergence effect.