Proteolytic processing of the L-type Ca 2+ channel alpha 1 1.2 subunit in neurons.

Background : The L-type Ca2+ channel Cav1.2 is a prominent regulator of neuronal excitability, synaptic plasticity, and gene expression. The central element of Cav1.2 is the pore-forming α 1 1.2 subunit. It exists in two major size forms, whose molecular masses have proven difficult to precisely determine. Recent work suggests that α 1 1.2 is proteolytically cleaved between the second and third of its four pore-forming domains (Michailidis et al ,. 2014). Methods : To better determine the apparent molecular masses (M R )of the α 1 1.2 size forms, extensive systematic immunoblotting of brain tissue as well as full length and C-terminally truncated α 1 1.2 expressed in HEK293 cells was conducted using six different region-specific antibodies against α 1 1.2. Results : The full length form of α 1 1.2 migrated, as expected, with an apparent M R of ~250 kDa. A shorter form of comparable prevalence with an apparent M R of ~210 kDa could only be detected in immunoblots probed with antibodies recognizing α 1 1.2 at an epitope 400 or more residues upstream of the C-terminus. Conclusions : The main two size forms of α 1 1.2 are the full length form and a shorter form, which lacks ~350 distal C-terminal residues. Midchannel cleavage as suggested by Michailidis et al . (2014) is at best minimal in brain tissue.