[Effects of subchronic aluminum exposure on long-term potentiation and activities of RAS and extracellular regulated protein kinases in rats].

Objective: To study the effects of subchronic aluminum exposure on LTP and activities of RAS and ERK in rats in vivo. Methods: 24 Wistar rats were randomly divided into control group、low-dose group、medium-dose group and high-dose group, and received saline (control group) or Al (mal) (3) (15 μmol、kg、30 μmol、kg or 45 μmol/kg) via intraperitoneal injection (i.p.) for 8 weeks, respectively. The fEPSP in CA1 region were recorded by field potentiation technique in vivo and the hippocampal activities of RAS and ERK were examined by ELISA. Results: The fEPSP amplitudes of the control group were 1.90±0.19, 1.64±0.15 and 1.54±0.08 at 1, 30 and 60 min after HFS, respectively. The fEPSP amplitudes of the low-dose group were 1.40±0.06 at 60 min, which represented a statistically significant decrease compared to the control group ( P <0.05) ; these values at 30min and 60min dropped to 1.33±0.20 and 1.12±0.07 in the medium-dose group ( P <0.05) and further decreased to 1.05±0.05 and 0.91±0.10 in the high-dose group ( P <0.05) . And the activity dose-dependent decreases were observed both in RAS and ERK: compared with the control group and the low-dose group, the activities of RAS and ERK of the medium-dose and high-dose group significantly decreased ( P <0.05) and compared with the medium-dose group, the activities of the high-dose group statistically dropped ( P <0.05) . Conclusion: RAS and ERK may be related to the suppression of LTP by subchronic aluminum exposure and the RAS-MAPK transduction pathway may be involved in the damage of learning and memory induced by aluminum.