A triple action chemosensor for Cu 2+ by chromogenic, Cr 3+ by fluorogenic and CN - by relay recognition methods with bio-imaging of HeLa cells.

A triple action chemosensor (R1) bearing a rhodamine and thiophene moiety was synthesized by a simple condensation reaction. The sensing behaviour and selectivity of the synthesized chemosensor toward metal ions were studied by UV-Vis and fluorescence spectroscopy. The chemosensor recognized Cu2+ and Cr3+ ions with significant changes in UV-Vis absorbance and fluorescence intensity. The results showed that Cr3+ induced greater fluorescence enhancement whereas Cu2+ ions bound strongly with the receptor by showing a strong absorption band at 554 nm but with weak fluorescence. A visible colour change was observed by the addition of Cu2+ and that colour change is due to the opening of the spirolactam ring triggered by the addition of Cu2+ ions. Job's plot analysis indicated a 1 : 2 and 1 : 1 binding stoichiometry between the chemosensor and Cr3+ /Cu2+ . Subsequently, the R1 - Cu2+ complex chemosensor was employed to detect CN- in the presence of different anions, such as F- , Cl- , Br- , I- , AcO- , H2 PO4 - , HSO4 - , NO3 - and - OH. In addition, the live cell imaging of HeLa cells using R1 and Cr3+ was demonstrated successfully.