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Genotyping Protocol for the Alpha-1 Antitrypsin (PiZ) Mouse Model.

Alisha M Gruntman
Methods in Molecular Biology 2017
The most common alpha-1 antitrypsin (AAT) mutant variant is a missense mutation (E342K), commonly referred to as PiZ. A transgenic mouse model exists that expresses the mutant human PiZ AAT gene. This protocol outlines the procedure used to extract DNA from and genotype AAT transgenic (PiZ) mice.

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