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Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos.
Scientific Reports 2017 July 26
In higher eukaryotic nuclei, DNA is periodically anchored to an extraction-resistant protein structure, via matrix attachment regions. We describe a refined and accessible method to non-subjectively, rapidly and reproducibly measure both size and stability of the intervening chromatin loops, and use it to demonstrate that malignant transformation compromises the DNA-nuclear matrix interface.
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