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A Simple Method to Induce Meiosis and Sporulation Semisynchronously in the Fission Yeast Schizosaccharomyces pombe .
Cold Spring Harbor Protocols 2017 September 2
Schizosaccharomyces pombe cells initiate a sexual differentiation program, which comprises meiosis and spore formation, on nitrogen starvation. This protocol describes a simple procedure to induce meiosis and sporulation semisynchronously in heterozygous diploid S. pombe cells. The procedure is appropriate for a variety of applications, including fluorescence-activated cell sorting (FACS) and northern and western blotting. Zygotic meiosis can also be induced by the same procedure, although less synchronously.
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