Isolation and Characterization of Aphidicolin Derivatives from Tolypocladium inflatum.

Inflatin G ( 1 ), a new aphidicolin analogue, together with seven known compounds inflatin A ( 2 ), inflatin B ( 3 ), aphidicolin ( 4 ), aphidicolin-17-monoacetate ( 5 ), gulypyrone A ( 6 ), pyridoxatin rotamers A ( 7 ) and B ( 8 ), were isolated from the ascomycete fungus Tolypocladium inflatum . Their structures were determined through NMR analyses and the circular dichroism data of the in situ formed [Rh₂(OCOCF₃)₄] complexes. Compounds 1 , 4 , 5 , 7 , and 8 showed modest cytotoxicity against four human cancer cell lines A549, CNE1-MP1, A375, and MCF-7.