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Aggregation-based colorimetric sensor for determination of prothioconazole fungicide using colloidal silver nanoparticles (AgNPs).
There is a growing interest in developing high-performance sensors monitoring fungicides, due to their broadly usage and their adverse effects on humans and wildlife. In the present study, a colorimetric probe has been proposed for detection of prothioconazole based on aggregation of unmodified silver nanoparticles (AgNPs). Under optimized condition, linear relationships between the concentration of prothioconazole and the absorbance ratio of A500 /A395 were found over the range of 0.01μg·mL-1 to 0.4μg·mL-1 with quantification limit as low as 1.7ng·mL-1 . Furthermore, AgNPs color change from yellow to pink-orange in presence of prothioconazole, indicates highly sensitive naked-eye colorimetric assay for quantifying prothioconazole in real applications. The proposed approach was successfully used for the determination of prothioconazole in wheat flour and paddy water sample.
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