The role of Notch1 genes in lung cancer A594 cells and the impact on chemosensitivity.

OBJECTIVE: Expression of Notch1 gene in lung cancer A549 cells was reduced using small interfering RNA (small interfering RNA, siRNA) and the effect of Notch1 gene on proliferation and chemo sensitivity of lung cancer A549 cells was studied.

MATERIALS AND METHODS: The Notch1 siRNA was transfected into A549 cells by liposome to inhibit the expressions of Notch1 gene in A549 cells. Real-time polymerase chain reaction (RT-PCR) and Western blot were used to detect the expression of Notch1 gene and protein. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to detect the proliferation of A549 cells. After transfection of Notch1-siRNA, cisplatin was added to each group at a concentration of 4 μg/ml, and cultured for 48 h. MTT assay and 4',6-diamidino-2-phenylindole (DAPI) staining was used to evaluate the change of apoptosis and sensitivity to chemotherapy.

RESULTS: Notch1 gene expression of A594 cells, detected by RT-PCR and Western blot was significantly reduced in transfected cells when compared with the control group (p<0.05). Inhibition of A594 cell proliferation was significantly decreased as detected by MTT (p<0.05), and the MTT assay and DAPI staining showed that Notch1 gene silencing can significantly improve the sensitivity of A549 cells to chemotherapeutic drugs.

CONCLUSIONS: The Notch1 siRNA can effectively inhibit the expression of Notch1 gene, inhibit the proliferation of lung cancer A549 cells and increase the sensitivity to chemotherapeutic drugs.